January 18 to January 19, 2021
The presented material provided an overview on the main concepts underlying the lipidomics approach. We started with a discussion on fatty-acid and lipid biosynthesis in plants, followed by introduction into the field of lipidomics analysis. Next, we presented an overview of the LC-MS-based lipdomics platform that is used to study the lipidome. Emphasis was put on the methods we are using for the annotation of some of the major classes of glycerolipids (i.e phosphatidylcholine (PC), phosphatidylethanolamines (PE), monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), triacylglycerol (TAG)). Aspects and techniques used for data analysis were introduced.
The workshop was divided into the following parts:
- Introduction to lipid biosynthesis in plants
- Principals and basics of LC-MS-based lipidomic analysis
- Annotation of glycerolipids and fatty acids
- Stable isotope labeling for flux analysis
- Statistical analysis and biological-data interpretation
This workshop is offered as part of the training activities of the RESIST project, and is free of charge.
Sign up for the workshop: Plant Lipidomics here, by January 15, 2021