January 18 to January 19, 2021
The presented material will provide an overview on the main concepts underlying the lipidomics approach. We will start with a discussion on fatty-acid and lipid biosynthesis in plants, followed by introduction into the field of lipidomics analysis. Next, we will present an overview of the LC-MS-based lipdomics platform we are using to study the lipidome. Emphasis will be put on the methods we are using for the annotation of some of the major classes of glycerolipids (i.e phosphatidylcholine (PC), phosphatidylethanolamines (PE), monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), triacylglycerol (TAG)). Aspects and techniques used for data analysis will be introduced.
The workshop is divided into the following parts:
- Introduction to lipid biosynthesis in plants
- Principals and basics of LC-MS-based lipidomic analysis
- Annotation of glycerolipids and fatty acids
- Stable isotope labeling for flux analysis
- Statistical analysis and biological-data interpretation
This workshop is offered as part of the training activities of the RESIST project, and is free of charge.
Sign up for the workshop: Plant Lipidomics here, by January 15, 2021